Cheeses

How to Make Cheese at Home Without Rennet

Nidhi Nangia
By Nidhi Nangia, Writer and researcher. Updated: June 26, 2026
How to Make Cheese at Home Without Rennet
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Cheese is a staple ingredient in many dishes around the world. Food items made with cheese are not only delicious but are also healthy and visually appealing! There are numerous types of cheeses, each offering its own unique taste and texture. However, when making cheese, one common factor is the use of rennet. Rennet contains a set of enzymes responsible for separating milk into solid curd. Typically, rennet is derived from the stomach of a calf, making it unsuitable for vegans.

If you love cheese but follow a vegan lifestyle, explore this oneHOWTO article on how to make cheese at home without rennet.

You may also be interested in: How to Make Cheese Curd at Home
Contents
  1. What is rennet?
  2. How can I make vegetarian rennet?
  3. Types of Cheese Made Without Rennet
  4. How to make cheese without rennet

What is rennet?

Before we delve into how to make cheese at home without rennet, it’s essential to understand what rennet is and why some people prefer to avoid it. Rennet is crucial for coagulating cheese, and chymosin is the primary enzyme involved, sourced from the fourth stomach lining of a new-born calf. This enzyme aids the calf in digesting its mother's milk efficiently. Rennet can also be obtained from piglets' stomachs, serving a similar digestive purpose. Inside the stomach, rennet curdles the milk, allowing it to remain longer in the digestive system for improved absorption.

The absence of chymosin can negatively affect a calf's health and digestion. Additionally, animal rennet can be costly and challenging to find. Therefore, even if you're not vegan but want to make cheese, learning to create cheese without rennet can be quite beneficial.

How can I make vegetarian rennet?

If you're looking to avoid animal rennet in cheese-making, you can create vegetarian rennet, which serves the same function of coagulating milk proteins. The difference lies in its origin, coming from microbes or vegetables. Here are some methods to make vegetarian rennet:

  • Vegetable rennet: Enzymes can be extracted from various plants to make vegetable rennet. Sources include fig leaves, safflower, melons, and wild thistles.
  • Microbial rennet: This type involves deriving enzymes from bacteria or fungi, followed by fermentation. However, microbial rennet may impart a bitter taste, making it less desirable for cheese-making.
  • Genetically engineered rennet: Fermentation-produced chymosin, or FPC, is another form of vegetarian rennet. It involves combining a calf's DNA gene with the DNA of bacteria, mold, or yeast, resulting in a GMO product. Most cheeses today are made using this type of rennet, which received FDA approval in 1990.
How to Make Cheese at Home Without Rennet - How can I make vegetarian rennet?

Types of Cheese Made Without Rennet

Here are several types of cheese that are made without rennet. These cheeses are typically not aged for long periods and are enjoyed fresh:

  • Cottage cheese: Coagulated with an acidic ingredient like vinegar, cottage cheese is a great rennet-free choice.
  • Cream cheese: A fresh cheese that is made without rennet, offering a smooth and creamy texture.
  • Mozzarella cheese: Brands such as Stella and Frigo produce mozzarella without rennet, ideal for dishes like paninis and pizzas.
  • Provolone cheese: Known for its robust flavor, provolone adds depth to sandwiches and casseroles.
  • Ricotta cheese: Organic ricotta cheese is light, sweet, and deliciously rennet-free.
How to Make Cheese at Home Without Rennet - Types of Cheese Made Without Rennet

How to make cheese without rennet

If rennet is unavailable or undesirable, acids like vinegar or citrus can be used as a substitute. Rennet activates between 85 to 140°F, reaching its peak potency at 105°F. When milk's acidity hits a pH of 4.6 and its temperature rises to 165°F, curd formation begins.

Follow these steps to replicate this process using citric acid or vinegar:

Ingredients required: milk, fresh lemon juice or distilled vinegar, and salt. Higher fat content in milk results in better cheese quality, but avoid UHT milk, as it is sterilized at 280°F, altering its protein structure and hindering separation.

How to make paneer cheese

Paneer is a mild cheese commonly used in Indian cuisines, made without rennet:

  1. Heat milk in a pan, ensuring it does not boil but only foams.
  2. Remove from heat.
  3. Gradually add lemon juice or vinegar, stirring continuously.
  4. As the acid is added, the milk will begin to curdle.
  5. Allow it to sit for 10-15 minutes, fully separating.
  6. Strain the curds using a cheesecloth in a sieve or colander.
  7. Squeeze out excess liquid.
  8. Spread the cheesecloth, sprinkle salt, wrap it tightly.
  9. Press the bundle between two plates.
  10. Let it dry for an hour. Enjoy your fresh paneer, ready to consume immediately without aging.

How to make queso fresco

This Latin-American cheese is similar to paneer, made by gradually adding distilled vinegar to warm milk. Many prefer vinegar, as lemon juice can impart a tangy flavor that not everyone enjoys.

Making cottage cheese

Cottage cheese involves draining but not pressing the cheese. To make it, heat buttermilk and milk with salt. The acidic buttermilk, once heated, separates the milk into curds. Higher fat buttermilk requires higher temperatures for curdling.

Making mascarpone

Mascarpone, a creamy cheese essential for tiramisu, is made using lemon juice and heavy cream. Due to its high fat content, a temperature of up to 190°F may be needed. Unlike milk, the liquid doesn't separate cleanly. This cheese naturally has a custard-like consistency and requires no squeezing.

How to Make Cheese at Home Without Rennet - How to make cheese without rennet

How to make homemade mozzarella without rennet

  1. Warm 2L of non-pasteurized/unboiled milk on a low flame, stirring continuously.
  2. Add 4 tbsp of white vinegar or lemon juice to curdle the milk.
  3. Curds should start to form. If insufficient, add an additional 1 tbsp of vinegar.
  4. Slowly gather all curds into a cheese ball.
  5. Remove the cheese ball and drain excess water. Place it into hot water, using the leftover water.
  6. Microwave the water until hot, then immerse the cheese ball, mixing continuously.
  7. Soak the cheese for 5-6 minutes, then squeeze out extra water using hands.
  8. Repeat soaking and squeezing 2 or 3 times.
  9. After soaking, immerse the cheese ball in ice-cold water for 1-2 minutes. Use tap water with ice cubes for maximum coldness.
  10. Repeat the cold water immersion process 2-3 times, squeezing out excess water each time.
  11. Wrap the cheese in plastic wrap and freeze for easy grating. Enjoy your homemade mozzarella!

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4 comments
Rating:
Kimmi
Can u add more vinegar to the whey u just used to make more.mozzarella? Or do u need to start all over? Thank u.
Rating:
John
How to make homemade mozzarella without rennet step 2 is missing??
Matthew Nesbitt (oneHOWTO editor)
Hi John,

Thank you for pointing this out, it is now fixed!
Rating:
Sampled The Recipe
Well my screen didn't say how much vinegar so but I used at least 1/3 cup for 1.5 gallons milk. Some of my milk was nonfat - not sure about that but I needed Lactaid milk in the mix for my lactose intolerance and nonfat was all they had. My yield for 1.5 gallons milk was only 3 little balls, each about the size of a tennis ball. I didn't have cheese cloth or flour sack, but it wasn't mentioned anyhow. But that meant my whey is not as clear, so not sure if it's any good. The cheese turned out bland so far, but it setting in the fridge. I don't mind bland cheese but if I'd known, I would have salted it. This took a lot longer than I thought, but I was dealing with a large quantity of milk and very bulky pans, so I couldn't tip them to strain them because I had to dip and pour. Probably wouldn't have started if I'd known it would take me hours. Am trying to figure how I can save time in the future. Thank you for helping me get started on first time cheese.
Kimmi
How to make homemade mozzarella without rennet
Warm 2L of non-pasteurized/unboiled milk on a low flame. Stir continuously.
Add 4 tbsp of white vinegar or lemon juice to split the milk.
Curds should start to form in the milk. If you do not see enough curds, you might need to add 1 tbsp more of vinegar.
Continuously and slowly gather all the curds into a cheese ball.
Take the cheese ball you have formed out and remove the excess water from it. Now the cheese has to be put into hot water, you can use the leftover water for this.
Microwave the water until it is hot and then place the cheese ball inside. Keep mixing.
You have to keep the cheese in this water for 5-6 mins. After that use your hands to squeeze out all the extra water from the cheese.
Now put it back in the water, take it out and again remove the extra water from it by squeezing it with your hands. You will repeat this process 2 or 3 times.
Once we've done that, we will soak the cheese ball in ice-cold water for 1 or 2 mins. We recommend you use tap water and ice cubes as we want the water to be very cold.
Take the cheese ball out, take out the excess water, dip it in again and repeat the process 2-3 times. Finally squeeze all excess water out.
Wrap the cheese in plastic foil and freeze it so it is easy to grate. And then, you are finally done!
Rating:
Me
does not tell me how to make mozzarella with no rennet
Lise
My comment is exactly as above. The only cheese you don't tell us how to make is mozzarella!

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How to Make Cheese at Home Without Rennet